Como hacer busquedas en el pubmed?

http://bitesizebio.com/2008/03/05/18-ways-to-improve-your-pubmed-searches/

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Genome Biology 2002, 3:research0034.1-0034.11doi:10.1186/gb-2002-3-7-research0034

Por favor lean este articulo. Todos aquellos que usen o vayan a usar RT PCR se beneficiaran de este. Valdria la pena estandarizar para cada linea celular
http://genomebiology.com/2002/3/7/research/0034

Un numero de acceso libre de bases de datos, muy util en Nucleic Acid Research
http://nar.oxfordjournals.org/content/vol36/suppl_1/index.dtl
y, en particular, la tabla de bases de datos
http://nar.oxfordjournals.org/cgi/content/full/gkm1037/DC1/1

1. Drop modesty (strive for excellence)
2. Prepare your mind (luck favors the prepared minds)
3. Age is important (research strategies should be different depending on your age, although I’m not sure I agree with that one)
4. Brains are not enough, you also need courage (I assume this means taking risks)
5. Make the best of your working condition (don’t let your environment limit you, but exploit the advantages you have)
6. Work hard and effectively (all the successful scientists I know are workaholics)
7. Believe and doubt your hypothesis at the same time (so be skeptical and accept data for what it is, but don’t give up on ideas before you test them)
8. Work on the important problems in your field (work on something interesting and relevant, no matter what the current trends are)
9. Be committed to your problem (because it might take a looong time to solve it)
10. Leave your door open (interact as much as you can with other people, you never know where the insights might come from)

De PLOS Computational Biology

List of contaminated cell lines

From Wikipedia, the free encyclopedia

This is a list of cell cultures which have been cross-contaminated and overgrown by other cells. A project is currently underway to enumerate and rename contaminated cell lines to avoid errors in research caused by misattribution (Masters, 2002). Estimates based on screening of leukemia–lymphoma cell lines suggest that about 15% of these cell lines are not representative of what they are usually assumed to be (Drexler et al., 2002).

Contaminated cell lines have been extensively used in research without knowledge of their true character. For example, most if not all research on the “endothelium” ECV-304 or the “megakaryocyte” DAMI cell lines has in reality been conducted on bladder carcinoma and erythroleukemia cells, respectively. Thus, all research on endothelium- or megakaryocyte-specific functions utilizing these cell lines has turned out to be worthless, except as a warning example.

There are two principal ways a cell line can become contaminated: cell cultures are often exchanged between research groups; if, during handling, a sample gets contaminated and then passed on, subsequent exchanges of cells will lead to the contaminating population being established, although parts of the supposed cell line are still genuine. More serious is contamination at the source: during establishment of the original cell line, some contaminating cells are accidentally introduced into the cultures, where they in time outgrow the desired cells. The initial testing, in this case, still suggested that the cell line is genuine and novel, but in reality, it has disappeared soon after being established and all samples of such cell lines are actually the contaminant cells. It requires lengthy research to determine the precise point where cell lines have become contaminated.

Este es un video interesante, por favor echenle un vistazo. Esta en ingles, pero lo principal aparece escrito. Es importante considerar este tipo de cuestiones para tener una carrera exitosa en la ciencia.
http://www.scivee.tv/pubcast/16261197

Este es un articulo interesante. Existe una correlacion entre la clase social de los padres y la mortalidad de los hijos despues de los 60 años, independientemente de la vida que hayan llevado.
Es decir, los hijos de padres de clase socioeconomica mas baja tienen mayor mortalidad, independientemente de si luego cambian de clase social, son adoptados, etc. La discusion es de si esta es genetico (lo cual seria catastrofico) o tiene que ver con el ambiente uterino.

link

Hola

Para los que han subclonado y hecho sus plasmidos, esta herramienta es muy util y facil de usar para hacer mapas.

http://wishart.biology.ualberta.ca/PlasMapper/index.html

Saludos

J.